Transformation of Cotton (Gossypium hirsutum L.) With Insect Resistant Gene by Particle Bombardment and Agrobacterium
نویسندگان
چکیده
منابع مشابه
Agrobacterium-mediated Transformation of Cotton (Gossypium hirsutum) Using a Synthetic cry1Ab Gene for Enhanced Resistance Against Heliothis armigera
Cotton (Gossypium hirsutum L.) is an important fiber crop in Iran, cultivated on 150000-200000 ha of land. In Iran the estimated loss due to the insect pest is more than 30%. Traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). In order to produce transgenic cotton resistance to in...
متن کاملtransformation of insect and herbicide resistance genes in cotton (gossypium hirsutum l.)
manipulation of different genes in crop plants to get desirable characters has become an important tool of plant biotechnology. in the current study, cotton variety mnh-786 was modified for its characteristics to show resistance against lepidopteran insects and herbicide by transformation of cry1ac+cry2a and gtgene cloned in a different cassette under 35s promoter. mature embryos of cotton mnh-...
متن کاملInfraspecific DNA methylation polymorphism in cotton (Gossypium hirsutum L.).
Cytosine methylation is important in the epigenetic regulation of gene expression and development in plants and has been implicated in silencing duplicate genes after polyploid formation in several plant groups. Relatively little information exists, however, on levels and patterns of methylation polymorphism (MP) at homologous loci within species. Here we explored the levels and patterns of met...
متن کاملStable Transformation of Saintpaulia ionantha by Particle Bombardment
Background: A highly efficient genetic transformation system is essential for a successful genetic manipulation of the African violet (Saintpaulia ionantha Wendl.). Objectives: Developing a particle bombardment-based genetic transformation system for the African violet. Materials and Methods: A local cultivar of the African violet from Guilan province was used for transformation experiments. Th...
متن کاملagrobacterium-mediated transformation of cotton (gossypium hirsutum) using a synthetic cry1ab gene for enhanced resistance against heliothis armigera
cotton (gossypium hirsutum l.) is an important fiber crop in iran, cultivated on 150000-200000 ha of land. in iran the estimated loss due to the insect pest is more than 30%. traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). in order to produce transgenic cotton resistance to in...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 1998
ISSN: 1028-8880
DOI: 10.3923/pjbs.1998.170.174